Overexpression of UHRF1 drives DNA hypomethylation and hepatocellular carcinoma

UHRF1 is an essential regulator of DNA methylation that is highly expressed in many cancers. Using transgenic zebrafish, cultured cells and human tumors, we demonstrate that UHRF1 is an oncogene. RNAseq was used to assess the variation in gene expression between control and experimental samples. Total small RNA from 2 batches of Tg(fabp10:has.UHRF1-GFP)High and age matched Tg(fabp10:nls-mCherry) control 5 dpf zebrafish livers was purified for preparation of high-throughput sequencing libraries.

泛素样含PHD和环指域1（UHRF1）是DNA甲基化的关键调控因子，在多种癌症中呈高表达。本研究通过转基因斑马鱼、体外培养细胞及人类肿瘤样本，证实UHRF1属于致癌基因。采用RNA测序（RNAseq）技术分析对照组与实验组样本的基因表达差异。我们从两批5 dpf（受精后第5天）的Tg(fabp10:has.UHRF1-GFP)高表达组与年龄匹配的Tg(fabp10:nls-mCherry)对照组斑马鱼肝脏中纯化总小RNA，用于制备高通量测序文库。