The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of SMC4 involved in replicative senescence

In this study, a label-free quantitative proteomic approach was employed to analyze the serial passaged human skin fibroblast （CCD-1079Sk） cells, within 3305 proteins quantified. Of which, 372 proteins were significantly changed in early passage(P6), middle passage (P12) and later passage (P21), with a time-dependent decrease or increase tendency. Then, of which, SMC4 was selected for further biological validation. The results confirmed that the expression of SMC4 was significantly down-regulated in a time-dependent manner in the subculture of human skin fibroblasts (HSFb) with Western Blot experiment.

本研究采用无标记定量蛋白质组学方法（label-free quantitative proteomic approach），对连续传代的人皮肤成纤维细胞CCD-1079Sk进行分析，共定量得到3305种蛋白质。其中，372种蛋白质在早期传代（P6）、中期传代（P12）及晚期传代（P21）中发生显著变化，呈现随传代时间推移而上调或下调的时间依赖性趋势。随后，本研究选取SMC4开展后续生物学验证。蛋白质免疫印迹（Western Blot）实验结果证实，在人皮肤成纤维细胞（HSFb，human skin fibroblasts）的连续传代培养过程中，SMC4的表达呈时间依赖性显著下调。