DataSheet_1_Responses of Soybean Genes in the Substituted Segments of Segment Substitution Lines Following a Xanthomonas Infection.zip

Bacterial blight, which is one of the most common soybean diseases, is responsible for considerable yield losses. In this study, a novel Xanthomonas vasicola strain was isolated from the leaves of soybean plants infected with bacterial blight under field conditions. Sequencing the X. vasicola genome revealed type-III effector-coding genes. Moreover, the hrpG deletion mutant was constructed. To identify the soybean genes responsive to HrpG, two chromosome segment substitution lines (CSSLs) carrying the wild soybean genome, but with opposite phenotypes following Xanthomonas inoculations, were used to analyze gene expression networks based on RNA sequencing at three time points after inoculations with wild-type Xanthomonas or the hrpG deletion mutant. To further identify the hub genes underlying soybean responses to HrpG, the genes located on the substituted chromosome segments were examined. Finally, a combined analysis with the QTLs for resistance to Xanthomonas identified 35 hub genes in the substituted chromosomal segments that may help regulate soybean responses to Xanthomonas and HrpG. Furthermore, two candidate genes in the CSSLs might play pivotal roles in response to Xanthomonas.

大豆细菌性疫病是最为常见的大豆病害之一，可造成显著的产量损失。本研究从田间条件下感染细菌性疫病的大豆植株叶片中，分离得到一株全新的维氏黄单胞菌（Xanthomonas vasicola）菌株。对该菌株的基因组进行测序分析，成功鉴定出III型效应因子编码基因。此外，本研究还构建了hrpG基因缺失突变体。为鉴定大豆中响应HrpG的基因，本研究选取两份携带野生大豆基因组、且在接种黄单胞菌（Xanthomonas）后呈现相反表型的染色体片段代换系（chromosome segment substitution lines, CSSLs），分别以野生型黄单胞菌及hrpG缺失突变体进行接种，并基于RNA测序技术对接种后三个时间点的基因表达调控网络展开分析。为进一步挖掘大豆响应HrpG过程中的核心基因，本研究对代换染色体片段上的编码基因进行了筛选。最后，结合抗黄单胞菌数量性状基因座（quantitative trait locus, QTL）进行联合分析，本研究在代换染色体片段中鉴定出35个核心基因，这些基因或可参与调控大豆对黄单胞菌及HrpG的应答过程。此外，该两份染色体片段代换系中的两个候选基因，可能在大豆应对黄单胞菌的过程中发挥关键调控作用。