Tracking the emergence of a novel genotype of Decapod hepanhamaparvovirus in shrimp using laser microdissection and next generation sequencing

Employing Laser Microdissection (LMD), we dissected cells containing intranuclear inclusion bodies pathognomonic for DHPV infection from histological sections. Whole Genome Amplification and NGS were used to generate five complete genomes of the novel DHPV isolate

本研究采用激光显微切割（Laser Microdissection, LMD）技术，从组织学切片中分离获取携带有DHPV感染特征性诊断核内包涵体的细胞。通过全基因组扩增（Whole Genome Amplification）与下一代测序（Next-Generation Sequencing, NGS），成功获得五株新型DHPV分离株的完整基因组。