Fig. 3e-f | Quantitative immunofluorescence for TDP-43 localization in motor neuron lines

This item is part of the <b>Figshare Project</b>:<br><b>Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolution</b><br>From <i>Data Availability Statement</i> for the forthcoming paper in <i>Nature Communications</i> entitled:<b>Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS</b>"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The <i>C9orf72</i>-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."--------------------------------------------[Item specific description:]Immunofluorescence microscopy of TDP-43 in in-vitro differentiated ALS motor neurons.These files are related to quantification of TDP-43 localization and phosphorylation in ALS motor neurons using the Opera Phenix high content imaging system. More information here on our imaging platform: https://www.kclwcic.co.uk/operaphenixSample meta information and plate layouts are available as XLSX and PDF files.The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package.<br>Information about the microscopy file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html<br><br>

本项数据属于<b>Figshare项目</b>：<br><b>单细胞分辨率下揭示FUS-和TARDBP-ALS中的早期线粒体功能障碍</b><br>来自即将发表于<i>Nature Communications</i>的论文《单细胞RNA测序揭示FUS-和TARDBP-ALS共有的运动神经元特异性早期线粒体功能障碍》的<i>数据可用性声明</i>“我们已将本研究中产生的所有原始及处理后的RNA测序数据存放在NCBI基因表达综合数据库（GEO，NCBI Gene Expression Omnibus），登录号为GSE226482。<i>C9orf72</i>-ALS bulk RNA测序数据直接取自该研究的作者。[本Figshare项目包含的内容：] “荧光Western印迹扫描图、共聚焦显微镜原始成像文件、Opera Phenix分析文件、qPCR数据集及Seahorse实验结果文件。”--------------------------------------------[本项数据具体描述：] 体外分化ALS运动神经元中TDP-43的免疫荧光显微镜成像数据。这些文件涉及使用Opera Phenix高内涵成像系统对ALS运动神经元中TDP-43的定位及磷酸化水平的定量分析。关于我们成像平台的更多信息请访问：https://www.kclwcic.co.uk/operaphenix 样本元信息及孔板布局以XLSX和PDF格式提供。文件格式为.czi，通过ZEN软件（Zeiss）生成。Zeiss推荐使用ImageJ及基于ImageJ的Fiji软件包。<br>关于显微镜成像文件格式CZI的信息可在此处获取：https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html<br><br>