Gene expression dynamics during regeneration of Eisenia fetida. Eisenia fetida

Earthworms show a wide spectrum of regenerative potential with certain species like Eisenia fetida, a terrestrial redworm, capable of regenerating more than two-thirds of their body while other closely related species, such as Paranais litoralis seem to have lost this ability. Earthworms belong to the phylum annelida, in which the genomes of the marine oligochaete Capitella telata, and the freshwater leech Helobdella robusta have been sequenced and studied. Herein, we report the de novo assembled transcriptome of Eisenia fetida (Indian isolate), along with an analysis of the transcriptomic changes during regeneration. We also used de novo assembled RNAseq data to identify genes that are differentially expressed during regeneration, both in the newly regenerating cells and in the adjacent tissue. Overall design: Gene Expression profiles of E.fetida, before and 15,20 or 30 days after cutting were generated by deep sequencing, in triplicate, using Illumina.

蚯蚓的再生潜能具有广泛的谱系分布，部分物种如赤子爱胜蚓（Eisenia fetida，一种陆生红蚯蚓）可再生自身超过三分之二的躯体，而与其亲缘关系较近的岸生拟仙女虫（Paranais litoralis）等物种似乎已丧失该再生能力。蚯蚓隶属于环节动物门（Annelida），该门类下海洋寡毛类动物 Capitella telata 与淡水水蛭 Helobdella robusta 的基因组已完成测序并得以研究。本研究报道了赤子爱胜蚓（Eisenia fetida，印度分离株）的从头组装转录组，并对其再生过程中的转录组变化展开分析。本研究同时借助从头组装的RNA测序数据，鉴定了再生过程中新生再生细胞及邻近组织内的差异表达基因。整体实验设计：采用Illumina平台开展深度测序，对赤子爱胜蚓切割前以及切割后15、20、30天的基因表达谱进行检测，每组设置三次生物学重复。