Effects of Nova1 Deletion on Gene Expression in Tissues and Cells

To elucidate the function of Nova1 in neurons, especially in inhibitory neurons, gene expression profiling analysis (RNAseq, TRAP-seq) was performed using Nova1 conditional knockout mice and their primary neurons. To analyze changes in gene expression in inhibitory neuron specific Nova1 knockout (cKO) mice, we performed RNA sequencing analysis of each brain region (hypothalamus, substantia nigra, periaqueductal gray, caudate putamen) and pituitary gland. In addition, TRAP-seq was performed on whole brains of neonatal mice to capture changes occurring in inhibitory neurons. Finally, primary neurons were used to assess the effects of Nova1 loss and the impact of overexpression of Impact, a Nova1 target gene.

为阐明Nova1在神经元（尤其是抑制性神经元）中的功能，本研究使用Nova1条件性敲除（conditional knockout，cKO）小鼠及其原代神经元，完成了涵盖RNA测序（RNAseq）与TRAP测序（TRAP-seq）的基因表达谱分析。为解析抑制性神经元特异性Nova1条件性敲除小鼠的基因表达变化，我们对多个脑区（下丘脑、黑质、中脑导水管周围灰质、尾状壳核）及垂体开展了RNA测序分析。此外，我们对新生小鼠全脑实施TRAP测序（TRAP-seq），以捕捉抑制性神经元内发生的表达变化。最后，本研究利用原代神经元，评估Nova1缺失的生物学效应，以及Nova1靶基因Impact过表达所产生的影响。