Systematic Study of in vitro Selection Stringency Reveals How to Enrich High-Affinity Aptamers

Library-immobilized SELEX was performed with an N30 stem-loop structured DNA library to isolate an aptamer that binds to the small molecule flunixin. Pools from various trials were sequenced.以N30茎环结构DNA文库为初始文库，开展文库固定化指数富集配体系统进化（SELEX）实验，筛选可特异性结合小分子氟尼辛的适配体；各轮试验所得的文库池均完成了测序分析。