Epigenomic profiling of human postmortem cortical samples

CUT&RUN was used to profile the epigenetic status of cells in human adult neurons (n=3) by measuring enirchment of H3K4me3 and H3K9me3 epigenetic marks over a non-targeting (IgG) control. CUT&RUN experiments (IgG, H3K4me3 and H3K9me3) were performed in nuclei of human adult NeuN+ nuclei (n=3)本研究采用CUT&RUN技术，以非靶向对照（IgG）为参照，通过检测H3K4me3与H3K9me3两种表观遗传标记的富集程度，对人类成人神经元（n=3）的细胞表观遗传状态进行表征。本次CUT&RUN实验（涵盖IgG、H3K4me3及H3K9me3三个实验组），在人类成人NeuN阳性细胞核（n=3）中完成。