Differentially expressed genes in wt and Nrf3-ko mouse keratinocytes before and after 24 h 100 mJ/cm2 UVB irradiation

We identified a pro-apoptotic function of Nrf3 in keratinocytes after UVB irradiation. To determine the underlying mechanism of action we wanted to compare the transcriptome of wt and Nrf3-ko mouse keratinocytes before and after 24 h 100 mJ/cm2 UVB irradiation Overall design: Wt and Nrf3-ko mice were mock-treated or UVB-irradiated and sacrificed 24 h later. Differentially expressed genes in the epidermis were identified using RNA-seq.

我们鉴定出Nrf3在紫外线B（UVB）照射后小鼠角质形成细胞（keratinocytes）中的促凋亡功能。为明确其潜在作用机制，本研究拟对比野生型（wild type, wt）与Nrf3基因敲除（Nrf3 knockout, Nrf3-ko）小鼠角质形成细胞在接受100 mJ/cm²紫外线B照射前及照射后24小时的转录组。实验设计概述：将野生型与Nrf3基因敲除小鼠分别施以假处理或紫外线B照射，并于处理/照射后24小时处死取材。通过RNA测序（RNA-seq）鉴定表皮（epidermis）组织中的差异表达基因（differentially expressed genes）。