Data for the Blood Proteoform Atlas:DS-76:PBMC - Transplant:Peripheral Blood

Human biology is tightly linked to proteins, yet most measurements do not precisely determine their full sequence and post-translational modifications. Here, we present the primary structures of 30,000 unique proteoforms expressed from 1,690 human genes across 21 cell types and plasma from human blood and bone marrow compiled in the Blood Proteoform Atlas (BPA). Our results indicate that while a given protein can be expressed across multiple cell types, the proteoform functions as a more specific indicator of differentiation. These results provide a better biochemical description of protein-level biology expressed through gene transcription and translation. We demonstrate the utility of the BPA by focusing on cell- and proteoform-specific signatures within 58 liver transplant recipients having healthy graft function or undergoing acute organ rejection or dysfunction.

人类生物学与蛋白质紧密关联，但当前多数检测手段无法精准测定蛋白质的完整序列及其翻译后修饰（post-translational modification）。本研究构建了血液蛋白质变体图谱（Blood Proteoform Atlas, BPA），收录了涵盖21种细胞类型以及人类血液与骨髓来源的血浆中、由1690个人类基因表达的30000种独特蛋白质变体（proteoform）的一级结构信息。研究结果表明，尽管某一蛋白质可在多种细胞类型中表达，但蛋白质变体可作为细胞分化的更特异性标志物。本研究为通过基因转录与翻译过程所介导的蛋白质层面生物学特征提供了更为精准的生化描述。我们以58名肝移植受者为研究对象，这些受试者要么移植物功能健康，要么正经历急性器官排斥或功能异常，并聚焦其中的细胞特异性与蛋白质变体特异性特征，以此验证了血液蛋白质变体图谱（BPA）的应用价值。