Expression data from c-Src inhibited osteoblasts. Mus musculus

The tyrosine kinase c-Src has a crucial role in maintaining osteoblast in a less differentiated status, as already described in the literature. We used microarrays to detail the global programme of gene expression in osteoblasts treated with a well known c-Src inhibitor (PP1). Overall design: Osteoblast primary cultures were isolated from calvaria of 7 days-old CD1 mice. These cell were starved and treated with vehicle (DMSO) or 2 micromolar PP1 for 24 hours. Then RNA was extracted and hybridizated on Affymetrix microarray.

酪氨酸激酶c-Src在维持成骨细胞处于低分化状态中发挥关键作用，该结论已在既往文献中得以阐明。我们借助微阵列（microarrays）技术，详细解析了经经典c-Src抑制剂PP1处理的成骨细胞的全局基因表达程序。
实验整体设计：原代成骨细胞培养物分离自7日龄CD1小鼠的颅盖骨。将这些细胞进行饥饿处理后，分别用溶剂对照（二甲基亚砜，DMSO）或2微摩尔浓度的PP1孵育24小时。随后提取总RNA，并在Affymetrix微阵列芯片上完成杂交。