Shared and distinct metabolic demands for innate and adaptive cytotoxic lymphocytes during viral infection. Shared and distinct metabolic demands for innate and adaptive cytotoxic lymphocytes during viral infection

Natural Killer (NK) cells and CD8+ T cells share many characteristics at steady state and during viral infection. In this study, we interrogate the metabolic pathways that fuel NK cell effector function in response to mouse cytomegalovirus (MCMV) infection. This dataset contains transcriptional profiling of wild-type and LDHA-deficient Ly49Hpositive NK cells at steady state and early after infection. Overall design: RNASeq was performed on wild-type or LDHA-deficient Ly49Hpositive NK cells harvested after 2 days post in vivo infection in a mixed bone chimera setting; 2-3 replicates per group and 20,000-60,000 cells per replicate.

自然杀伤细胞（Natural Killer cells）与CD8+ T细胞在稳态及病毒感染过程中共享诸多共性特征。本研究旨在解析驱动NK细胞在小鼠巨细胞病毒（mouse cytomegalovirus，MCMV）感染后发挥效应功能的代谢通路。本数据集收录了稳态条件下及感染早期的野生型与乳酸脱氢酶A（Lactate Dehydrogenase A）缺陷型Ly49H阳性NK细胞的转录组谱数据。整体实验设计：本研究在混合骨髓嵌合模型中，对体内感染2天后收获的野生型及LDHA缺陷型Ly49H阳性NK细胞开展RNASeq测序；每组设置2-3个生物学重复，每个重复的细胞数为20000至60000个。