Forkhead box Q1-regulated transcriptome in human breast cancer cells. Forkhead box Q1-regulated transcriptome in human breast cancer cells

RNA-seq was performed to identify novel mechanistic targets of FoxQ1 in human breast cancer cells using empty vector-transfected control (EV) and FoxQ1 overexpressing SUM159 cells. We found that interleukin-1α and interleukin-8 are direct transcriptional targets of FoxQ1. Overall design: Examination of altered genes expression in empty vector-transfected control (EV) and FoxQ1 overexpressing SUM159 cells (n=3 for each)

为鉴定人乳腺癌细胞中FoxQ1的新型机制靶点，本研究以空载体转染对照（EV）与FoxQ1过表达的SUM159细胞为模型开展RNA测序（RNA-seq）实验。本研究发现，白细胞介素-1α（interleukin-1α）与白细胞介素-8（interleukin-8）为FoxQ1的直接转录靶点。实验整体设计：对空载体转染对照（EV）与FoxQ1过表达的SUM159细胞（每组n=3）的差异基因表达情况进行检测。