Gene expression data from MCF-7 cells treated with Lacciac Acid A

Lacciac Acid A was indentified as an inhibitor of DMNT1. MCF-7 cells were treated with Lacciac Acid A (200 uM) for 5 days. Changes in gene expression were identified by using Affymetrix Human gene ST1.0 arrays. We used microarrays to determine global changes in gene expression upon treatment with Lacciac Acid A an inhibitor of DMNT1. MCF-7 cells near mid passage were treated with Lacciac Acid A for 5 days. RNA was extracted and hybridized on Affymetrix microarrays. Treated and untreated cells were from the same cell passage. Two sample sets from the same passage and the third set from the previous passage were used.

本研究鉴定出Lacciac Acid A为DMNT1的抑制剂。将MCF-7细胞以200 μM的Lacciac Acid A处理5天，通过Affymetrix Human Gene ST1.0芯片检测基因表达变化。本研究借助微阵列技术，旨在明确经DMNT1抑制剂Lacciac Acid A处理后细胞内基因表达的全局变化。取处于传代中期的MCF-7细胞，以Lacciac Acid A处理5天；提取总RNA后，在Affymetrix微阵列芯片上进行杂交。处理组与未处理的对照组细胞均来自同一细胞传代批次。本次实验共设置三组样本：其中两组取自同一传代批次，第三组取自前一代次的细胞。