MAPK p38 and NFkB activity dynamics in macrophages upon innate immune stimulation

Dataset (.mat structure) containing single cell p38 and NFkB activity dynamics in macrophages (iMPDMs) upon innate immune stimulation (LPS, TNF, P3C4, CpG) (1 h baseline + app 8 h stimulation).

Two biological replicates per stimulation condition are included. 6 doses per stimulus are included.

Signaling dynamics are quantified using live cell microscopy followed by automated image analysis by MACKtrack.

Datasets include dynamic features ('metrics'), MACKtrack quantification outputs ('measure'), info on experimental (esp. dose), tracking, and quantification parameters, and filtering outcomes ('info').

本数据集为.mat格式结构文件，包含巨噬细胞（iMPDMs）在受到天然免疫刺激（脂多糖(LPS)、肿瘤坏死因子(TNF)、P3C4、CpG）后的单细胞p38与核因子κB(NFkB)活性动态变化数据，实验周期为1小时基线观测阶段+约8小时刺激处理阶段。

每个刺激条件设置两个生物学重复，每个刺激物包含6个剂量梯度。

信号通路动态变化通过活细胞显微成像量化，后续采用MACKtrack工具完成自动化图像分析。

本数据集涵盖动态特征指标（'metrics'）、MACKtrack量化输出结果（'measure'）、实验相关参数信息（涵盖剂量设置、细胞追踪与量化参数）以及数据筛选结果（'info'）。