pSHDY#_PpetE:BCD2:mVenus

Autonomous shuttle plasmid pSHDY# (w/o mobAY25F point mutation) used for Cu2+-induced overexpression of a codon optimized mVenus reporter fluorophore in the cyanobacterium Synechocystis sp. PCC 6803.Basic plasmid backbone precursor of pSHDY (doi: https://doi.org/10.1101/757948) and pSHDY*in.The synthetic expression module contains promoter PpetE from Synechocystis sp. PCC 6803 and the bicistronic design BCD2 (doi: 10.1038/nmeth.2404).

本载体为缺失mobAY25F点突变的自主穿梭质粒pSHDY#，可用于在蓝细菌集胞藻（Synechocystis sp. PCC 6803）中经Cu²+诱导过表达密码子优化的mVenus报告荧光蛋白。本质粒是pSHDY（DOI：https://doi.org/10.1101/757948）与pSHDY*in的基础质粒骨架前体。其合成表达模块包含源自集胞藻Synechocystis sp. PCC 6803的PpetE启动子，以及双顺反子设计元件BCD2（DOI：10.1038/nmeth.2404）。