Single-cell reference cell state map of early mouse development. Single-cell reference cell state map of early mouse development

Ontogeny describes the orchestrated assembly of a complex multicellular organism from a single totipotent zygote. As the embryo grows, proliferating cells become restricted in their fate to realize one of many encoded phenotypes, which are subsequently maintained through the combined action of cell-type specific transcription factors and broadly expressed regulatory machinery. Here, we provide a reference map of mouse development through gastrulation and early organogenesis, which can be used to explore the partitioning of early lineages and discrete cell states. Overall design: Single-cell RNAseq data was collected on whole early mouse embryos. Each experiment consists of 4-10 replicates of developmental stages 6.5, 7.0, 7.5, 8.0, 8.5 for wild-type sequenced with 10X Genomics single-cell RNA sequencing system.

个体发生（Ontogeny）指由单个全能受精卵协调有序地构建形成复杂多细胞生物的过程。随着胚胎发育，增殖细胞的发育命运逐渐受到限定，仅能表达基因组编码的众多表型中的一种；后续这些表型将通过细胞类型特异性转录因子与广谱表达调控机制的协同作用得以维持。本研究提供了小鼠原肠胚形成至早期器官发生阶段的发育参考图谱，可用于探究早期谱系的分配与离散细胞状态的界定。
实验设计概述：本数据集采集了完整早期小鼠胚胎的单细胞RNA测序（Single-cell RNAseq）数据。每个实验包含针对野生型小鼠6.5、7.0、7.5、8.0、8.5五个发育阶段的4-10次生物学重复，均采用10X Genomics单细胞RNA测序系统完成测序。