Chain elongating, mesophilic community - Raw sequence reads

Communities fermenting mixtures of acetic acid and ethanol, in the pressence of different gas mixtures (N2, N2:CO2, and N2:H2) at mesophilic conditions (37 C) and a pH of 6.8. The fermentations also exhibited competing reactions such as acetogenesis and sulfate reduction. The inocula were previous vials operating at the same conditions. The original inoculum was sludge obtained from Lyngby-Taarbaek Wastewater Treatment Plant, which was heat-preteated to remove methanogens.Chain elongation implies the use of ethanol to elongate short-chain fatty acids such as acetic acid into longer fatty acids such as butyric, caproic, or caprylic acid. However ethanol can be also used in competing pathways, reducing the yield of longer-chain fatty acids. The goal of the study was to evaluate the impact the presence of H2 and CO2 would have on competing reactions to reverse beta chain elongation.

本数据集对应的实验体系为：以乙酸与乙醇混合液为底物的微生物群落，在中温（37℃）、pH 6.8的条件下，采用氮气（N₂）、N₂:CO₂、N₂:H₂三种不同气体氛围开展厌氧发酵。发酵过程中伴随产乙酸作用与硫酸盐还原等竞争反应。所用接种物为预先在相同条件下运行的发酵瓶菌种，原始接种物取自Lyngby-Taarbaek污水处理厂的污泥，经热处理以去除产甲烷菌。
链延长反应是利用乙醇将乙酸等短链脂肪酸延伸为丁酸、己酸或辛酸等长链脂肪酸，但乙醇也可参与其他竞争代谢途径，进而降低长链脂肪酸的产率。本研究旨在探究H₂与CO₂的存在对竞争反应的影响，以逆转β-链延长过程。