New xylose transporters support the simultaneous consumption of glucose and xylose in Escherichia coli. New xylose transporters support the simultaneous consumption of glucose and xylose in Escherichia coli

RNA-seq libraries were constructed for six samples, including AE1.0, AE2.0, PS1.0, PS2.0, REV1.0, REV2.0 . For preparation of RNA samples, Co-utilization were assessed using AM1 mineral salt media supplemented with glucose and xylose at each concentration of 50 g·L-1. Seed culture were grown overnight (16 h) in AM1 medium supplemented with 2% sugar. For each experiment, E. coli was grown at 37 ℃ in a pH-controlled fermentation vessel with the initial cell density at OD550nm of 0.1.Total RNA for samples were isolated using TRIzol reagent (Invitrogen, Grand Island, USA), then used for high-throughput RNA sequencing. The 150-nt paired-end RNA-seq libraries were generated commercially at Novogene Biotechnology Co. Ltd (Tianjin, China) by using Illumina’s Hiseq X Ten platform (Illumina, San Diego, USA). Overall design: mRNA profiles of six samples were generated by deep sequencing, using Illumina’s Hiseq X Ten platform

本研究为6个样本（包括AE1.0、AE2.0、PS1.0、PS2.0、REV1.0、REV2.0）构建了RNA测序（RNA-seq）文库。在RNA样品制备环节，本研究采用补充了终浓度均为50 g·L⁻¹的葡萄糖与木糖的AM1矿物盐培养基，对底物共利用情况进行评估。种子培养液在添加2%糖类的AM1培养基中过夜培养16小时。每次实验中，大肠杆菌（E. coli）于37℃、pH可控的发酵罐中培养，初始细胞密度设定为OD550nm=0.1。采用TRIzol试剂（美国格兰艾兰的Invitrogen公司）提取各样本的总RNA，随后用于高通量RNA测序。150 nt双端RNA测序文库由中国天津的诺禾致源生物科技有限公司（Novogene Biotechnology Co. Ltd）基于Illumina Hiseq X Ten测序平台（美国圣迭戈的Illumina公司）商业构建完成。整体实验设计：采用Illumina Hiseq X Ten平台通过深度测序，获取6个样本的mRNA表达谱。