MCJ deficient mice respond to anti-TNF treatment

In order to evaluate response to anti-TNF treatment mcj deficient mice were administered 2% of DSS ad libitum in drinking water for 6 days, followed by three days of recovery period. Then, mice were divided into two groups per genotype, receiving only one intraperitoneal administration of Inflectra (7.5 mg/kg), a Infliximab biosimilar (IFX+) proven to be effective for induction and maintenance of remission in moderate to severe UC. The mouse/human chimeric monoclonal IgG1 antibody to TNF, was provided from day 3 to 6 during DSS administration while the second group received saline solution (IFX-). Colon samples were collected at the end of the experimental period (day 9).

为评估小鼠对抗肿瘤坏死因子（Tumor Necrosis Factor，TNF）治疗的应答效果，研究人员令mcj缺陷型小鼠自由饮用含2%葡聚糖硫酸钠（Dextran Sulfate Sodium，DSS）的饮用水，持续6天，随后设置3天的恢复期。随后按基因型将小鼠分为两组：一组仅接受单次腹腔注射英夫利昔单抗生物类似药Inflectra，给药剂量为7.5 mg/kg；该生物类似药（IFX+）已被证实可有效诱导并维持中重度溃疡性结肠炎（Ulcerative Colitis，UC）的临床缓解。靶向TNF的鼠-人嵌合IgG1单克隆抗体于DSS给药期间的第3至6天完成给药，而另一组则给予生理盐水（IFX-）。实验周期结束时（第9天）收集结肠样本。