Effect of intracanal diode laser application and low-level laser therapy on CGRP change

Abstract: The aim of this study was to investigate the effect of a placebo, intracanal diode laser application, and low-level laser therapy (LLLT) on the change of the total amount of calcitonin gene-related peptide (CGRP) in the gingival crevicular fluid (GCF) (split-mouth study design). GCF sampling was performed on a contralateral tooth and experimental tooth (root canal-treated tooth) of thirty-nine patients. The patients were divided into three groups (n = 13), as follows: placebo (mock laser application), intracanal laser application, and LLLT. GCF sampling was repeated at the same sites (experimental and control teeth) one week after root canal treatment. The data were analyzed using the Pearson's correlation analysis and the independent-samples t-tests (p=0.05). In the placebo group, the total CGRP level changes in the GCF before and after treatment was significantly higher for experimental teeth than for control teeth (p<0.05). However, there was no significant difference between experimental and control teeth in the intracanal laser application and LLLT groups (p > 0.05). Intracanal laser application and low-level laser therapy have immunomodulation effects linked to the modulation of the total amount of CGRP in the GCF.

摘要：本研究旨在探讨安慰剂、根管内二极管激光应用与低能量激光疗法（low-level laser therapy, LLLT）对龈沟液（gingival crevicular fluid, GCF）中降钙素基因相关肽（calcitonin gene-related peptide, CGRP）总量变化的影响，本研究采用分口研究设计。
研究纳入39例患者，分别采集其对侧牙齿与实验牙（根管治疗患牙）的龈沟液样本。将患者分为3组（每组n=13），具体分组如下：安慰剂（模拟激光照射）组、根管内激光应用组及低能量激光疗法组。根管治疗1周后，于相同位点（实验牙与对照牙）重复采集龈沟液样本。采用皮尔逊相关分析与独立样本t检验（p=0.05）对数据进行分析。
在安慰剂组中，实验牙治疗前后龈沟液中CGRP总量的变化幅度显著高于对照牙（p<0.05）。而在根管内激光应用组与低能量激光疗法组中，实验牙与对照牙的CGRP总量变化无显著统计学差异（p>0.05）。
根管内激光应用与低能量激光疗法具有免疫调节作用，该作用与龈沟液中CGRP总量的调控相关。