Metabolic Profiles in Soft Palate Tissue in Chronic Intermittent Hypoxia Induced Rats

Six-week-old male Sprague-Dawley rats (170±2 g) were randomly assigned to two groups, each consisting of 5 rats, and housed in polycarbonate cages. Rats were provided with standard food and water and maintained on a 12-h light/dark cycle. The CIH group rats were exposed to intermittent hypoxia using plastic cages equipped with intermittent hypoxia devices for 8 h per day, with CIH being performed during the 12-h light cycle to align with the animals' sleep cycle. CIH rats were subjected to CIH for 28 consecutive days in 8-hour daily cycles from 9:00 am to 5:00 pm. During each cycle, the internal oxygen concentration was reduced from 21% to 8.5% over 4 min and maintained at this level for 2 min. The oxygen concentration was then rapidly restored to 21% for 2 min and maintained at this level for 1 min. Animals in the control group were housed in the same chamber but were provided with a constant flow of 21% oxygen. The soft palate tissue samples collected from rats were stored at a temperature of –80 ℃.

将6周龄雄性斯普拉格-道利（Sprague-Dawley）大鼠（体重170±2 g）随机分为两组，每组5只，饲养于聚碳酸酯笼具中。大鼠自由采食标准饲料与饮水，维持12小时明/暗交替光照周期。慢性间歇性低氧（Chronic Intermittent Hypoxia, CIH）组大鼠采用配备间歇性低氧装置的塑料笼进行间歇性低氧暴露，每日暴露8小时，且CIH处理安排在12小时光照周期内，以匹配动物的睡眠节律。具体处理为：连续28天每日于上午9:00至下午5:00的8小时周期内实施CIH。每个暴露周期中，舱内氧浓度于4分钟内从21%降至8.5%，并维持该浓度2分钟；随后快速恢复至21%，维持2分钟后再保持1分钟。对照组大鼠饲养于相同舱室，但持续通入流量恒定的21%氧气。采集大鼠软腭组织样本，保存于-80℃环境中。