Complete Genome of a Clinical Isolate of Salmonella enterica serovar Paratyphi A. Salmonella enterica serovar Paratyphi A strain AKU12601 whole genome

The 4.5 Mb genome of a clinical isolate of Salmonella enterica serovar Paratyphi A was completely sequenced using capillary techniques and subsequently checked using Illumina/Solexa re-sequencing. Annotation and sequence comparison with other serovar Paratyphi A and Typhi genomes identified several pseudogenes that had been overlooked in prior genome annotations, and showed that 66 pseudogenes are common to both serovars by virtue of conservation (38), recent recombination (6) and independent mutation (22), highlighting distinct mechanisms that may have contributed to the phenotypic convergence of these genetically distinct human-restricted pathogens.

本研究采用毛细管测序技术完成了一株临床分离的甲型副伤寒沙门氏菌（Salmonella enterica serovar Paratyphi A）的4.5 Mb基因组全序列测定，随后通过Illumina/Solexa重测序技术对测序结果进行了验证。对该基因组进行注释并与其他甲型副伤寒沙门氏菌及伤寒沙门氏菌（Typhi）的基因组开展序列比对后，研究团队鉴定出了此前基因组注释中被遗漏的多个假基因；分析显示这两种血清型共拥有66个共享假基因，其中38个源于序列保守性、6个源于近期重组事件、22个源于独立突变，这一结果揭示了可能促成这两种遗传背景迥异的人源性限制性病原体表型趋同的不同分子机制。