Polysorbate 80-fed, carboxymethylcellulose-fed and control microbiota DataSet

[Description of methods used for collection/generation of data] The three colon reactors (R1, R2 and R3) of the BFBL gut simulator were inoculated (1%) with child (5-7 years old) pooled fecal sample. After the microbiota stabilization, polysorbate 80 or carboxymethylcellulose were added into the BFBL simulator at increasing concentrations (1, 3 and 5 g/L in case of polysobrbate 80 or 0.3, 1, 3 and 5 g/L in case of carboxymethylcellulose), three times per day during 1 week for each concentration. In addition, another run without additives feeding (control) was carried out for the same period of time to assess changes due to the experimental conditions. During the experimental set up, samples were collected every day from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet and supernatant were stored separately. Bacterial genomic DNA from the pellet was extracted according to the manufacturer’s instruction of the EZNA® Bacterial DNA Kit (Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads.

[数据收集/生成所用方法的描述] BFBL肠道模拟器的三个结肠反应器（R1、R2和R3）接种了（1%）5-7岁儿童混合粪便样本。微生物群稳定后，向BFBL模拟器中添加浓度递增的聚山梨酯80（polysorbate 80）或羧甲基纤维素（carboxymethylcellulose）：聚山梨酯80的浓度为1、3和5 g/L，羧甲基纤维素的浓度为0.3、1、3和5 g/L；每种浓度下每天添加三次，持续一周。此外，还进行了另一组无添加剂的对照实验，持续相同时间，以评估实验条件引起的变化。实验期间，每天从三个结肠反应器中采集样本，在4°C下以11200×g离心10分钟，然后分别储存沉淀物和上清液。根据EZNA®细菌DNA试剂盒（Omega Biotek）的制造商说明，并使用FastPrep设备进行机械裂解（Bio 101 FastPrep FP120，Savant Instruments），从沉淀物中提取细菌基因组DNA。DNA样本被送往诺禾致源欧洲公司（Novogene Europe，英国剑桥），对通过341F（5′-CCTAYGGGRBGCASCAG-3′）和806R（5′-GGACTACNNGGGTATCTAAT-3′）引物扩增的16S rRNA基因V3-V4区域进行测序。PCR产物在Illumina双端测序平台（NovaSeq 6000，Illumina）上测序，生成250 bp的双端原始读数。