Response of Foodborne pathogens to novel antimicrobial interventions

The adaptability of meat-associated pathogens under stress raises concerns for consumers, healthcare professionals and industry stakeholders. Salmonella enterica serovar Typhimurium NCTC14028 and Campylobacter jejuni ATCC11168 were treated with sublethal variations of proposed antimicrobial interventions proposed for use in the meat production industry. These interventions include plasma functionalised water (glow and spark-system created), LED ultraviolet light, and a novel antibacterial marinade composed of a citrus juice and essential oil component. After treatment, RNA was isolated using a QiAgen RNEasy Micro RNA extraction kit, and converted to cDNA utilising an Oxford Nanopore cDNA-PCR sequencing kit protocol (SQK-PCS111). This library was indexed according to NEBNext Ultra II Q5 Index protocol, and sequenced on an Illumina MiSeq utilising a 150-cycles V3 flow cell.

肉类相关病原菌在胁迫环境下的适应性，引发了消费者、医护人员以及行业利益相关方的广泛担忧。本研究采用拟用于肉类生产行业的多种亚致死剂量抗菌干预手段，对肠炎沙门氏菌鼠伤寒血清型（Salmonella enterica serovar Typhimurium）NCTC14028与空肠弯曲杆菌（Campylobacter jejuni）ATCC11168进行处理。所涉抗菌干预手段包括：由辉光与火花系统制备的等离子体功能化水、LED紫外光，以及一种由柑橘汁与精油成分复配的新型抗菌腌料。处理完成后，使用凯根（Qiagen）RNeasy Micro RNA提取试剂盒分离总RNA，并按照牛津纳米孔（Oxford Nanopore）cDNA-PCR测序试剂盒操作流程（SQK-PCS111）将RNA反转录为cDNA。随后按照NEBNext Ultra II Q5索引试剂盒操作流程对该测序文库进行索引标记，并使用Illumina MiSeq测序平台搭配150循环V3测序流动槽完成测序。