TFEB and TFE3 are required for efficient cellular response against beta-coronavirus [ChIP-Seq]. TFEB and TFE3 are required for efficient cellular response against beta-coronavirus [ChIP-Seq]

The transcription factors EB and E3 (TFEB and TFE3) promote lysosomal biogenesis and autophagy in response to a variety of stress conditions. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the ß-coronaviruses family. Recent studies have shown that ß-coronaviruses use lysosomes to egress the cell. The aim of this work is to analyze if ß-coronavirus infection promotes TFEB/3 activation and to evaluate the contribution of these transcription factors to the cellular response upon viral infection. Overall design: Analysis of TFE3 binding to promoter of genes under Mouse Hepatitis Virus (MHV) infection in HeLa-mCC1a cells

转录因子EB与E3（TFEB、TFE3）可响应多种应激条件，促进溶酶体生物发生与自噬过程。严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）隶属于β冠状病毒科。近期研究表明，β冠状病毒可借助溶酶体完成细胞出胞。本研究旨在分析β冠状病毒感染是否可促进TFEB/TFE3的活化，并评估此类转录因子在病毒感染过程中对细胞应答的贡献。整体实验设计：在HeLa-mCC1a细胞中，分析小鼠肝炎病毒（MHV）感染状态下TFE3与基因启动子的结合情况。