Genome sequencing of Pseudomonas taiwanensis VLB120 and genome-reduced strain GRC2

We engineered solvent-tolerant Pseudomonas taiwanensis VLB120 to yield streamlined chassis strains with higher growth rates and biomass yields, enhanced solvent tolerance, and improved process performance. In total, the genome was reduced by up to 10%. The generated P. taiwanensis GRC strains enlarge the repertoire of streamlined chassis with enhanced key performance indicators, making them attractive hosts for biotechnological applications.

本研究对耐溶剂台湾假单胞菌（Pseudomonas taiwanensis）VLB120进行基因工程改造，成功获得精简型底盘菌株。该类菌株具备更高的生长速率与生物量得率、更强的溶剂耐受性，且工艺性能得到显著优化。经改造后，菌株基因组的总删减幅度可达10%。本研究构建的台湾假单胞菌P. taiwanensis GRC菌株，扩充了具备优化关键性能指标的精简底盘菌株资源库，使其成为极具应用价值的生物技术宿主。