nanopore sequencing analysis of integron gene cassettes in sewages and soils

this study applied nanopore sequencing for the variable regions for class 1 integrons to explore the contents of gene cassette pool from sewage and manure-amended soil, characterizing the gene cassettes associated with antibiotic resistance and understanding the impact of anthropogenic activities on the integron-mediated ARG dissemination.

本研究针对1类整合子（Class 1 Integrons）的可变区采用纳米孔测序（Nanopore Sequencing）技术，旨在探究污水与有机肥改良土壤（Manure-Amended Soil）中的基因盒库（Gene Cassette Pool）组成，解析与抗生素耐药性（Antibiotic Resistance）相关的基因盒，并阐明人为活动（Anthropogenic Activities）对整合子介导的抗生素耐药基因（Antibiotic Resistance Genes, ARG）传播的影响。