Expression data from normal breast tissues and invasive primary breast carcinoma tissues. Homo sapiens

The role of lymphangiogenesis in tumor metastasis remains unclear. This study addressed this issue in lymphatic endothelial cells (LECs) derived from primary invasive breast cancer specimens. LECs were isolated by laser capture microdissection and gene expression was profiled by microarray analysis. Overall design: To analyze global gene expression patterns in LECs derived from primary invasive breast tumors, we compared gene expression profiles between fresh human normal mammary glands (N4 and N5) and primary invasive breast tumors (BL1 and B14). Specimens were dissected, and rapid immunochemical staining of lymphatic-specific markers, FLT-4 and LYVE-1, was used to identify lymphatic endothelium. Homogenous LECs were separated directly in situ by laser capture microdissection. Intact RNA was extracted, and the RNA samples were used for subsequent GeneChip probe microarrays.

淋巴管生成（lymphangiogenesis）在肿瘤转移中的作用至今仍未明确。本研究针对该科学问题，对源自原发性浸润性乳腺癌标本的淋巴管内皮细胞（lymphatic endothelial cells, LECs）展开了相关研究。研究人员通过激光捕获显微切割技术分离得到LECs，并通过微阵列分析完成基因表达谱的检测。整体实验设计：为分析原发性浸润性乳腺癌来源LECs的全局基因表达模式，本研究将新鲜正常人乳腺组织（N4、N5）与原发性浸润性乳腺癌组织（BL1、B14）的基因表达谱进行了对比。对标本进行解剖后，通过淋巴管特异性标志物FLT-4与LYVE-1的快速免疫化学染色，完成对淋巴管内皮的鉴定。随后通过激光捕获显微切割技术，直接在原位分离得到纯度均一的LECs。提取完整RNA后，将RNA样本用于后续的GeneChip探针微阵列实验。