2009-2010 Outbreak: Salmonella enterica subsp. enterica serovar Typhimurium Genome Sequencing

To better understand the genomic relationship of Salmonella enterica subsp. enterica serovar Typhimurium isolates sharing the JPXX01.0014 pulse field gel electrophoresis (PFGE) type, we sequenced and analyzed the whole genome shotgun assemblies of five clinical isolates that were isolated across the United States between 2009 and 2010. Three additional isolates possessing the same PFGE type collected from celery in 2002, raw pecans in 2009 and from California pond water in 2009 were also sequenced. Comparison of all of these sequences with the seven completed S. Typhimurium genomes clearly demonstrate that all of these isolates are nearly identical to S. Typhimurium strain 14082, which has been utilized in laboratory experiments for over two decades.

为明确携带JPXX01.0014脉冲场凝胶电泳（PFGE）型别的鼠伤寒沙门氏菌（Salmonella enterica subsp. enterica serovar Typhimurium）分离株的基因组关联特征，本研究对2009至2010年间在美国全境分离获得的5株临床分离株开展全基因组鸟枪测序，并对其组装序列进行分析。另有3株携带相同PFGE型别的分离株亦完成测序，它们分别于2002年自芹菜中、2009年从生山核桃内以及2009年于加利福尼亚池塘水体中分离得到。将上述所有序列与7株已完成组装的鼠伤寒沙门氏菌基因组进行比对分析后，结果清晰表明：所有受试分离株均与已在实验室实验中应用二十余年的鼠伤寒沙门氏菌菌株14082高度同源。