Microbial activities associated with human intestinal mucosal biofilms change mRNAs and microRNAs to promote carcinogenesis in mice [miRNA]

Disrupted interactions between host and intestinal bacteria are implicated in the development of colorectal cancer (CRC). However, the functional impacts of these inter-kingdom interactions remain poorly defined. To examine this interplay, we performed small RNA sequencing on the stool of from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues. revealed a group of significant differentially expressed miRNAs specific to BT compared to BX associated ApcMin/+;Il10-/- mice and several miRNAs that correlated with bacterial genera abundances. Our findings suggest complex interactions within bacterial communities affecting host-derived miRNA and CRC development. Small RNA sequencing on stool samples from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues.

宿主与肠道菌群的相互作用失调与结直肠癌（colorectal cancer, CRC）的发生发展密切相关。然而，这类跨界相互作用的功能效应仍未得到充分阐明。为探究这一相互作用，我们对无菌（germ-free, GF）以及定植了生物膜阳性人结直肠癌肿瘤（biofilm-positive human CRC tumor, BT）或生物膜阴性健康组织（biofilm-negative healthy, BX）来源菌群的限菌（gnotobiotic）ApcMin/+;Il10-/-小鼠的粪便样本进行了小RNA测序（small RNA sequencing）。测序结果显示，与定植BX菌群的ApcMin/+;Il10-/-小鼠相比，定植BT菌群的小鼠存在一组特异性差异表达的microRNAs (miRNAs)，同时还鉴定出若干与细菌属丰度存在相关性的miRNAs。本研究结果表明，菌群群落内部存在复杂的相互作用，可影响宿主来源miRNA的表达以及结直肠癌的发生发展。我们对无菌（germ-free, GF）以及定植了生物膜阳性人结直肠癌肿瘤（biofilm-positive human CRC tumor, BT）或生物膜阴性健康组织（biofilm-negative healthy, BX）来源菌群的限菌（gnotobiotic）ApcMin/+;Il10-/-小鼠的粪便样本进行了小RNA测序（small RNA sequencing）。