Transcriptional profiling of cadmium effect in human adipocytes

Adipose tissue is a potential site of accumulation of toxicants, including trace elements, which can have an impact on tissue functionality. To investigate the molecular perturbations induced by Cd in mature adipocytes, we treated Adipose Tissue-derived human mesenchymal stem cells (AD-hMSCs) with 1microM cadmium for 48h and we used microarrays to detail the global program of gene expression. We examined 2 experimental groups: control cells (C) and cadmium treated cells (Cd). For each experimental group we analysed the transcriptional profile of 3 biological replicates.

脂肪组织是包括微量元素在内的有毒污染物的潜在蓄积位点，可对组织功能产生影响。为探究镉（Cd）对成熟脂肪细胞所诱导的分子扰动，我们以1μM镉处理脂肪组织来源的人间充质干细胞（AD-hMSCs）48小时，并通过微阵列（microarrays）解析全局基因表达程序。本实验设置两组：对照组（C）与镉处理组（Cd）。针对每组实验，我们均对3次生物学重复的转录谱进行了分析。