The examination of the nephroprotective effect of montelukast sodium and N-acetylcysteine ın renal ıschemia with dimercaptosuccinic acid imaging in a placebo-controlled rat model

Abstract Purpose To determine the nephroprotective effect of NAC and Montelukast Sodium administration against the development of renal damage associated with long warm renal ischemia. Methods Twenty-seven rats were randomly divided into 3 study groups, which received NAC, montelukast and placebo, and 3 rats were included in the sham-treated control group. Medications were given 3 days before the procedure. DMSA renal scintigraphy was performed before and after surgery. The right renal pedicle was occluded for 45 min to induce ischemia and then subjected to reperfusion for 6 h (I/R groups). Results On pathological examination, the mean pathological scores of the montelukast and NAC groups were significantly lower than those of the placebo group. (p <0.05). In biochemical examination, significant differences were found in all parameter levels between the placebo group and the montelukast and NAC groups. (p <0.05) When postoperative DMSA renal scintigraphy measurements and renal function levels were compared, significant differences were found between the montelukast and NAC groups and the placebo and sham groups. Conclusion The administration of NAC and montelukast sodium was seen to have a nephroprotective effect against the development of renal damage associated with warm renal ischemia.

摘要 目的 探讨N-乙酰半胱氨酸（NAC）与孟鲁司特钠给药对长时间温热性肾缺血相关肾损伤进展的肾脏保护作用。方法 将27只大鼠随机分为3个给药组，分别予以NAC、孟鲁司特钠及安慰剂干预，另设3只大鼠作为假手术对照组。于术前3天开始给药。分别于手术前后行二巯基丁二酸（DMSA）肾显像检查。通过阻断右侧肾蒂45分钟诱导肾缺血，随后再灌注6小时（缺血-再灌注组，即I/R组）。结果 病理检查显示，孟鲁司特钠组与NAC组的平均病理评分均显著低于安慰剂组（p<0.05）。生化检查结果表明，安慰剂组与孟鲁司特钠组、NAC组的所有参数水平均存在显著差异（p<0.05）。对比术后DMSA肾显像检测结果与肾功能水平，孟鲁司特钠组、NAC组与安慰剂组、假手术组之间均存在显著差异。结论 给予NAC与孟鲁司特钠可对温热性肾缺血相关肾损伤的进展发挥肾脏保护作用。