Transmission of xanthomonads to Medicago truncatula seeds. Medicago truncatula

Plant pathogenic bacteria disseminate and survive through transmission to and by seeds of hosts and non-hosts plants. To investigate the interaction between xanthomonads and developing seeds of Medicago truncatula, plants at the flower bud stage were spray inoculated until runoff with xanthomonads suspensions. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed on seeds to characterize the molecular dialogue between Xanthomonas campestris pv. campestris in an incompatible situation with M. truncatula seeds and Xanthomonas alfalfae pv. alfalfae in a compatible situation at two developmental time points (16 and 32 days atfter pollination (DAP). Overall design: Six-condition experiment, 16dap_Mock versus 16dap_Xaa, 16dap_Mock versus 16dap_Xcc, 32dap_Mock versus 32dap_Xaa, 32dap_Mock versus 32dap_Xcc. Biological replicates: 6 controls (16dap_Mock, 32dap_Mock), 12 treatments (16dap_Xaa, 16dap_Xcc, 32dap_Xaa, 32dap_Xcc), independently grown and harvested. One replicate per array.

植物病原细菌可通过寄主与非寄主植物的种子完成传播与定殖存活。
为探究黄单胞菌属（Xanthomonads）与蒺藜苜蓿（Medicago truncatula）发育中种子的互作关系，本研究对花芽期的蒺藜苜蓿植株喷施黄单胞菌悬浮液，直至菌液在叶面开始下淌。
本研究采用蒺藜苜蓿NimbleGen基因芯片，对授粉后16天（days after pollination, DAP）与授粉后32天（DAP）两个发育时间点的蒺藜苜蓿种子开展转录组分析，以解析两种互作体系下的分子对话：一是野油菜黄单胞菌野油菜致病变种（Xanthomonas campestris pv. campestris, Xcc）与蒺藜苜蓿种子的不亲和互作，二是苜蓿黄单胞菌苜蓿致病变种（Xanthomonas alfalfae pv. alfalfae, Xaa）与蒺藜苜蓿种子的亲和互作。
整体实验设计：本实验包含6个实验条件，共设置4组两两对比，分别为16dap_Mock vs 16dap_Xaa、16dap_Mock vs 16dap_Xcc、32dap_Mock vs 32dap_Xaa、32dap_Mock vs 32dap_Xcc。
生物学重复：共包含6份对照样本（16dap_Mock、32dap_Mock）与12份处理组样本（16dap_Xaa、16dap_Xcc、32dap_Xaa、32dap_Xcc），所有样本均独立培养并收获，每块芯片对应一个生物学重复。