Summary of generation of <i>Leishmania</i> hybrids using different gDNA.

Transfection efficiency was calculated by dividing the number of colonies by the number of transfected cells. The relative abundance of stable and episomal transformants were determined on clones by CHEF, followed by Southern blot as described in the Methods using the respective drug resistance marker as probe.转染效率通过集落数除以转染细胞数计算得到。通过钳位均匀电场脉冲场凝胶电泳（Contour-clamped Homogeneous Electric Field, CHEF）对克隆进行分析，随后参照实验方法部分所述，以相应药物抗性标记作为探针进行Southern印迹（Southern blot），以此测定稳定型转化子与游离型转化子的相对丰度。