Root-specific transcriptional profiling of contrasting rice genotypes in response to salinity stress. Oryza sativa Indica Group

Analysis of root gene expression of salt-tolerant genotypes FL478, Pokkali and IR63731, and salt-sensitive genotype IR29 under control and salinity-stressed conditions during vegetative growth. Results provide insight into the genetic basis of salt tolerance in indica rice. Keywords: stress response Overall design: Seedlings were cultured in sand and irrigated with a nutrient solution for 22 d (salt-treated) and 30 d (control) after germination, respectively. Salinity treatment was applied by adding NaCl and CaCl2 (5:1 molar concentration) in two steps over a period of 3 days (final electrical conductivity: 7.4 dS m-1) to prevent osmotic shock. All plants were harvested on day 30.

本研究对营养生长阶段的耐盐基因型FL478、Pokkali、IR63731及盐敏感基因型IR29，在对照与盐胁迫条件下的根部基因表达情况进行了分析。研究结果可为籼稻（indica rice）的耐盐遗传基础解析提供参考。关键词：胁迫响应（stress response）。实验设计：种子萌发后，采用砂基培养并浇灌营养液的方式培育幼苗，盐处理组培育22天，对照组培育30天。盐胁迫处理通过分两步加入摩尔浓度比为5:1的NaCl与CaCl2完成，处理周期为3天，最终电导率为7.4 dS·m⁻¹，以避免渗透冲击。所有植株均于培养第30天收获。