Transcription profile of infiltrating monocytes derived from 3D liver cancer cell line spheroids. Transcription profile of infiltrating monocytes derived from 3D liver cancer cell line spheroids

To study the molecular mechanism of tumor associated macrophages (TAMs), the RNA of infiltrating monocytes derived from in vitro liver cancer spheroid model were performed. The results indicated that the cholesterol metabolism-related genes enriched in the infiltrating monocytes, which presented the M2-like TAM phenotypes. Overall design: In this study, the infiltrating monocytes isolated from 3D liver cancer cell lines consisted of infiltrating monocytes in HepG2 spheroids and the infiltrating monococytes in PLC/PRF/5 spheroids. The freshly isolated monocytes from PBMCs were control. The gene expressions were performed on NanoString nCounter Human Myeloid Innate Immunity Panel.

为研究肿瘤相关巨噬细胞（Tumor Associated Macrophages, TAMs）的分子机制，本研究对体外肝癌球体模型来源的浸润性单核细胞的RNA进行了表达检测。结果显示，浸润性单核细胞中富集了胆固醇代谢相关基因，且呈现出M2样肿瘤相关巨噬细胞表型。
实验设计：本研究所分离得到的浸润性单核细胞来自3D肝癌细胞系，具体包括HepG2球体中的浸润性单核细胞与PLC/PRF/5球体中的浸润性单核细胞；以从外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）中新鲜分离的单核细胞作为对照。本研究的基因表达检测采用NanoString nCounter Human Myeloid Innate Immunity Panel完成。