Inflammatory bowel disease. Homo sapiens

Samples for microarray analysis were derived from terminal ileum and colonic tissues from probands with Crohn´s disease and Ulcerative Colitis and control patients, respectively. IBD tissue biopsies from non-inflamed regions 10 cm distant from pathological areas were selected. To minimize inter-individual differences in gene expression and to enrich for IBD-specific transcriptional events, 2.5 µg of total RNA from terminal ileum and colon transversum from four individuals of each patient and control group were used for pooling. Keywords = IBD Keywords = Crohn´s disease Keywords = Ulcerative Colitis Keywords: other

本基因芯片分析（microarray analysis）所用样本，分别采集自克罗恩病（Crohn´s disease）受试患者、溃疡性结肠炎（Ulcerative Colitis）受试患者与健康对照者的回肠末端（terminal ileum）及结肠组织（colonic tissues）。
研究选取了距病变区域10 cm处的非炎症区域的炎症性肠病（IBD）组织活检标本。
为最小化基因表达的个体间差异并富集炎症性肠病特异性转录事件，本研究将每组患者及对照组各4名个体的回肠末端与横结肠（colon transversum）总RNA（total RNA）各2.5 µg进行混合，用于后续实验。
关键词：炎症性肠病（IBD）、克罗恩病（Crohn´s disease）、溃疡性结肠炎（Ulcerative Colitis）、其他。