Dorsal dependent CBP binding on a genome wide scale

We find a high concordance between the binding of the Drosophila transcription factor Dorsal and the co-activator CBP during early embryogenesis. This relationship was furter examined by comparing CBP distribution in Drosophila embryos derived from wt and mutant flies lacking intranuclear Dorsal (gd7). Our data suggests a specific involvemet of CBP in initiating early dorsoventral patterning, but not in anterioposterior. Overall design: CBP ChIP seq of 2-4 hours old Drosophila embryos derived from w1118 (wild-type) or gd7 homozygous mutant mothers

我们发现，果蝇（Drosophila）转录因子背蛋白（Dorsal）与共激活因子CBP在早期胚胎发生过程中的结合具有高度一致性。我们通过对比野生型（wt）以及缺失核内背蛋白的突变果蝇（gd7）所产生的果蝇胚胎中CBP的分布情况，进一步验证了这一关联。我们的数据表明，CBP特异性参与早期背腹模式建成，但并不参与前后轴模式建成。实验整体设计：对来自w1118（野生型（wild-type））或gd7纯合突变母本的2-4小时龄果蝇胚胎进行CBP染色质免疫共沉淀测序（ChIP-seq）。