The exposed proteomes of Brachyspira hyodysenteriae and B. pilosicoli

Applying a shotgun proteomics approach, we built a unique database of Brachyspira hyodysenteriae and B. pilosicoli peptides and proteins. A total of 148 LC-MS/MS runs were conducted on an Orbitrap instrument, and more than 29000 different peptides in cell supernatants and partially digested cell suspensions of 3 strains of B. hyodysenteriae and 2 strains of B. pilosicoli were identified. Overall, 16970 and 15493 peptide sequences, which pointed to 1625 and 1338 different protein accessions, were identified in B. hyodysenteriae and B. pilosicoli, respectively. The large-scale characterization of peptides and proteins in the extracellular medium as well as of the exposed proteins on the bacterial surface in these species provides a unique collection of interrelated data on the molecules involved in the interaction of these bacteria with their environment.

本研究采用鸟枪法蛋白质组学（shotgun proteomics）策略，构建了猪痢疾短螺旋体（Brachyspira hyodysenteriae）与结肠短螺旋体（B. pilosicoli）的专属肽与蛋白质数据库。本研究于轨道阱（Orbitrap）质谱仪上完成总计148次液相色谱-串联质谱（LC-MS/MS）分析，对3株猪痢疾短螺旋体和2株结肠短螺旋体的细胞上清液及部分消化细胞悬浮液中的肽段进行鉴定，共识别出29000余种不同肽段。其中，猪痢疾短螺旋体中鉴定得到16970条肽序列，对应1625个不同蛋白质登录号（protein accessions）；结肠短螺旋体中则鉴定得到15493条肽序列，对应1338个不同蛋白质登录号。对上述两种短螺旋体细胞外介质中的肽与蛋白质，以及细菌表面暴露蛋白进行大规模表征，可获得一套关于这些细菌与环境互作相关分子的独特关联数据集。