Down-regulation of SFRP1 in a mammary epithelial cell line

Microarray data to accompany Gauger et al. (2009) Cancer Cell International paper A treated X untreated design was used whereby immortalized human mammary epithelial cell lines (TERT cells) were modified to stably express an siRNA that knocked down SFRP1 expression. These "treated" cells were labeled with Cy5 and four replicate harvests were hybridized to Agilent 4by44k whole genome microarrays, along with a Cy3 labeled untreated control. The untreated control consisted of 4 pooled harvests of the parent cell line in normal growth conditions at 60-80% confluence.

本数据集为配套Gauger等人2009年发表于《Cancer Cell International》的研究论文的微阵列（Microarray）数据。实验采用处理组vs未处理组的实验设计：将永生化人乳腺上皮细胞系（TERT细胞）进行基因修饰，使其稳定表达可敲低SFRP1表达的小干扰RNA（siRNA）。将上述‘处理组’细胞以Cy5荧光基团标记，取4次生物学重复样本，与Cy3标记的未处理对照组样本共同杂交于安捷伦（Agilent）4×44k全基因组微阵列芯片。未处理对照组为亲本细胞系在正常培养条件（细胞汇合度60%~80%）下的4次混合收获样本。