A comparison of eukaryote community composition in four contrasting sediments in the Celtic Sea

As part of the NERC funded Shelf Seas Biogeochemistry program, microbial structure, diversity and abundance was compared in four contrasting sediment types, from mud to sand. Samples were collected before (March 2015) and immediately after a spring diatom bloom (May) and also again in late summer (August). These data are 16S rRNA and 18S rRNA gene sequence sets from the four sediment types, and from the three sampling time points. Relative abundance and composition was determined using Illumina MiSeq (MrDNA, TX, USA). The V1–V3 region of 16S rRNA was amplified using the PCR primers 27F (Vergin et al., 1998) and 519Rmod (Andreotti et al., 2011) using the PCR and sequencing conditions described in Tait et al. (2015). For 18S rRNA genes, the primers Euk7F and Euk570R were used.

本研究作为英国自然环境研究理事会（NERC）资助的陆架海生物地球化学项目的一部分，对覆盖泥质至砂质范围的4种不同沉积类型中的微生物群落结构、多样性与丰度开展了对比分析。样本分别于春季硅藻水华暴发前（2015年3月）、暴发后即刻（5月）以及夏末（8月）三个时间点完成采集。本数据集包含上述4种沉积类型及3个采样时间点对应的16S rRNA与18S rRNA基因序列数据集。研究采用Illumina MiSeq测序平台（美国德克萨斯州MrDNA实验室）完成了样本相对丰度与群落组成的测定。其中，16S rRNA基因的V1–V3高变区采用PCR引物27F（Vergin等，1998）与519Rmod（Andreotti等，2011）进行扩增，扩增及测序流程参照Tait等（2015）发表的方法执行；18S rRNA基因扩增则采用引物Euk7F与Euk570R。