CIL:1439

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:1440.

将家兔腰大肌骨骼肌纤维固定于恒定长度，并通过甘油浸泡诱导其进入僵直状态。随后将纤维置于Heuser型冷冻压片机中，通过与液氦冷却铜块接触实现快速冷冻，再在Balzers 400型冷冻断裂仪中完成冷冻蚀刻处理。之后以24°倾角采用铂-碳（Pt-C）对样品进行旋转复型，并在加速电压为100 keV的JEOL 100CX2型透射电子显微镜下进行观测成像，以68000倍的放大倍率将图像记录于柯达（Kodak）4489型胶片上。以±5°的倾角采集立体图像对，使用尼康（Nikon）Coolscan 9000ED扫描仪，以20微米的像素间距对胶片进行数字化扫描。该立体图像对的编号为CIL:1440。