Effects of Dietary Supplementation with Hydroponic Wheat Seedlings on Rumen Fermentation, Meat Quality, Amino Acid and Fatty Acid Contents, and Rumen Bacterial Diversity in Sheep

This study investigates the structural and functional dynamics of rumen microbiota in Hu sheep (Ovis aries) fed hydroponic wheat seedlings (HWS) through 16S rRNA sequencing. By characterizing microbial diversity, taxonomic composition, and metabolic potential under graded HWS substitution (DM), the research aims to elucidate how this sustainable fodder modulates rumen fermentation patterns, nitrogen utilization efficiency, and fibrolytic activity. The findings will establish mechanistic links between HWS-induced microbial shifts and zootechnical outcomes, addressing critical gaps in precision nutrition strategies for ruminants. As hydroponic systems gain traction in arid regions like Xinjiang, this work provides actionable insights into optimizing feed conversions while reducing reliance on conventional water- and land-intensive forages, thereby advancing eco-efficient livestock production paradigms.

本研究采用16S rRNA测序技术，探究饲喂水培小麦苗（hydroponic wheat seedlings, HWS）的湖羊（Ovis aries）瘤胃微生物群的结构与功能动态变化。本研究设置以干物质（dry matter, DM）为基准的梯度HWS替代日粮，对该日粮模式下的微生物多样性、分类学组成及代谢潜能进行表征，旨在阐明该可持续饲草如何调控瘤胃发酵模式、氮利用效率与纤维分解活性。本研究结果将建立HWS诱导的微生物群落变迁与动物生产性能之间的机制关联，填补反刍动物精准营养策略领域的关键研究空白。鉴于水培系统在新疆等干旱地区的应用日益广泛，本研究可为优化饲料转化率、降低对传统高水耗高土地占用饲草的依赖提供可落地的理论指导，进而推动生态高效型畜牧生产范式的发展。