Comparative analysis of gene expression in coral Acropora tenuis exposed to a UV filter or heat stress

RNA-seq was performed to reveal differences in coral responses to UV filter and heat stress. Fragments of adult stony coral Acropora tenuis were exposed to four concentrations of benzophenone-3 (BP-3) ranged from 0.38 to 2.7 mg/L or heat stress (31°C) for 96 h. RNA was extracted from each fragment (n = 6) and RNA-seq was performed on the DNBSEQ-G400 platform (2 � 200-bp).

本研究采用RNA测序（RNA-seq）技术，旨在解析珊瑚对紫外线过滤剂与热胁迫的响应差异。将成年石珊瑚纤细鹿角珊瑚（Acropora tenuis）的组织片段，分别暴露于四种浓度的二苯甲酮-3（BP-3）环境中（浓度范围为0.38~2.7 mg/L），或置于31℃的热胁迫条件下，处理时长均为96小时。从每个珊瑚组织片段（每组含6个生物学重复样本）中提取总RNA，随后在DNBSEQ-G400测序平台上开展RNA测序，测序策略为2×200 bp双端测序。