Investigation of the gene expression profile of LYVE-1-positive macrophages

LYVE-1-positive macrophages were observed to be closely spatially associated with the developing lymphatic vasculature. The role of this population of macrophages in the embryo is uncharacterised. We used microarray analyses to investigate which genes are differentially regulated between LYVE-1-positive and LYVE-1-negative macrophages Macrophage populations were isolated from cell suspensions prepared from digested embryonic day (E) 15.5 skin and prepared for RNA extraction and hybridization on Affymetrix microarrays.研究观察到LYVE-1阳性巨噬细胞（LYVE-1-positive macrophages）与发育中的淋巴管脉管系统存在紧密的空间关联。该巨噬细胞亚群在胚胎中的功能尚未被阐明。我们采用基因微阵列分析，旨在探究LYVE-1阳性与LYVE-1阴性巨噬细胞间的差异调控基因。巨噬细胞群从经酶消化处理的胚胎第15.5天（E15.5）皮肤组织制备的细胞悬液中分离获得，随后用于RNA提取及Affymetrix基因芯片杂交实验。