Salmonella enterica serovar Typhimurium strain 14028s transcriptome response to soil media

Summary: Salmonella enterica serovar Typhimurium strain 14028s transcriptome response to DS soil suspension (DS) and suspension of autoclaved DS soil (DA) compared to minimal medium (MM). Purpose: Salmonella mRNA profile, when grown in different media was compared to minimal medium to reveal environment specific transcriptional changes. Methods: mRNA profiles were generated using Illumina HiSeq in triplicates. The sequences were analysed using Bowtie2 followed by Cufflinks. mRNA profiles of Salmonella were generated using Illumina HiSeq in triplicates.

摘要：鼠伤寒沙门氏菌（Salmonella enterica serovar Typhimurium）菌株14028s在DS土壤悬液（DS）、高压灭菌DS土壤悬液（DA）与基础培养基（minimal medium, MM）中的转录组响应特征。目的：将沙门氏菌在不同培养基中生长时的mRNA表达谱与基础培养基（MM）进行比对，以揭示环境特异性的转录变化。方法：先后两次采用Illumina HiSeq平台完成三次生物学重复，以生成沙门氏菌的mRNA表达谱；序列分析先通过Bowtie2进行序列比对，随后采用Cufflinks完成后续处理。