Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to specific neurons isolation and advanced understanding their functions. In this study, the whole brains of female SD rats aged PND-25, 35 and 45were performed 10 µm serial tissue sections transversely to expose ARC regions (bregma: -2.52 to 2.92 mm, interaural: 6.08 to 6.48 mm) according to Allen Brain Atlas and processed by spatial transcriptomics sequencing.

鉴定与青春期相关的细胞组成，并解析不同细胞间独特的转录特征，有助于特异性神经元的分离以及对其功能的深入认知。本研究以出生后第25、35、45天的雌性斯普雷格-道利（Sprague-Dawley, SD）大鼠为研究对象，参照艾伦脑图谱（Allen Brain Atlas），对其全脑进行横向10 μm连续组织切片以暴露弓状核（Arcuate Nucleus, ARC）区域，其定位坐标为：前囟点（bregma）-2.52~2.92 mm，耳间距（interaural）6.08~6.48 mm，随后对样本开展空间转录组测序（spatial transcriptomics sequencing）。