Gene expression analysis of TGFß1-stimulated fibroblasts to assess anti-fibrotic effects of the bisubstrate-like NNMT inhibitor II559

Fibroblast activation drives tissue fibrosis and is often mediated by TGFß1 signaling. Human fibroblasts were pre-treated with the NNMT inhibitor II559 for 12 hours, followed by TGFß1 stimulation for 72 hours. Transcriptomic profiling revealed that II559 prevented pro-fibrotic gene expression, providing a resource for understanding how NNMT inhibition modulates fibroblast activation and fibrotic remodeling. Overall design: Human dermal fibroblasts(n=3) were treated with TGF-ß1 (10 ng/mL) in the presence or absence of II559 (30 µM). Following 72 h incubation, RNA was harvested and subjected to RNA-seq.

成纤维细胞激活可驱动组织纤维化，该过程通常由转化生长因子β1（TGF-β1）信号通路介导。研究人员将人成纤维细胞用烟酰胺N-甲基转移酶（NNMT）抑制剂II559预处理12小时，随后采用TGF-β1刺激72小时。转录组分析显示，II559可抑制促纤维化基因的表达，本数据集为解析NNMT抑制如何调控成纤维细胞激活及纤维化重塑提供了研究资源。整体实验设计：选取3例人真皮成纤维细胞，分别在添加或不添加30 μM II559的条件下，以10 ng/mL的TGF-β1进行处理。孵育72小时后收集RNA并进行RNA-seq。